Thermo Fisher – Glacios - Cryogenic Transmission Electron Microscope (TEM):

The Glacios is a 200kV transmission electron microscope. The Glacios includes a sample handling robot called the autoloader, which keeps the samples stored in ultra-high vacuum conditions at a temperature of -193 deg C. The system is equipped with a Falcon 4i direct detector camera system, enabling the capture of up to 320 frames per second internally and 20 frames per second to the customer at 64M pixel per frame. The direct detector is an electron counting detector, which provides the ability to count nearly every electron that strikes the detector. This high signal-to-noise provides the ability to image fragile proteins before they are destroyed by the electron beam. The system has automated software for 3-dimensional tomography applications for cells and tissues, as well as single-particle automated software for 3-dimensional near atomic resolution structure determination. Additionally, the Glacios is equipped with a CetaD 16M pixel detector and automated software for performing electron diffraction imaging experiments called microED. MicroED is a technique whereby micro protein crystals are imaged in diffraction mode to determine 3-dimensional protein structures to below 1 Angstrom. This system will primarily be used to determine 3-dimensional protein structures down to the approximately the 3 Angstrom level of resolution on general proteins. The Glacios can solve 3-dimension protein structures at resolutions of up to 1.8Angstroms. in ideal samples. The Glacios system is available to all University of California students, staff, and faculty as well as external institutions both private and public. Access is dependent upon completion of the electron optics training as well as hand on Glacios provided by UCSB CryoEM staff. Access to the facility and staff is managed by our scheduler software.